Journal: Journal of applied microbiology

Article Title: Assessment of stress tolerance acquisition in the heat-tolerant derivative strains of Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus rhamnosus GG.

doi: 10.1111/jam.12520

Figure Lengend Snippet: Figure 2 Representative 2D gels of the Bif. animalis subsp. lactis BB-12 and Lact. rhamnosus GG strains. Proteins spots identified in the mass spectrometry analysis are labelled. BB-12, Bifidobacterium animalis subsp. lactis BB-12; LGG, Lactobacillus rhamnosus GG; MM, molecular mass; pI, isoelectric point.

Article Snippet: In this study, we used two-dimensional differential ingel electrophoresis (2D-DIGE) coupled with mass spectrometry, together with reverse transcriptase-quantitative PCR (RT-qPCR), to assess the heat response in the two most commonly used probiotic strains, Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus rhamnosus GG (ATCC 53013), and to compare them with their previously obtained heat-tolerant derivatives (BB-12 HS and LGG HS) (du Toit et al. 2013).

Techniques: Mass Spectrometry

Journal: Antioxidants

Article Title: Exploring Beneficial Properties of Haskap Berry Leaf Compounds for Gut Health Enhancement

doi: 10.3390/antiox13030357

Figure Lengend Snippet: Bacterial strains used in the research, along with an indication of their origin.

Article Snippet: Bifidobacterium animalis , Probiotic bacteria , ATCC BAA-2848.

Techniques: Bacteria

Journal: Nutrients

Article Title: The Influence of Probiotic Lactobacilli on COVID-19 and the Microbiota

doi: 10.3390/nu16091350

Figure Lengend Snippet: Information extracted from the literature regarding article specifics and corresponding research findings for lactobacilli as probiotic strains.

Article Snippet: Laterza, L. et al., 2023 [ ] , Italy , 19 , Missing information , 19 post-COVID-19 patients , Lactobacillus paracasei BP07, Lactobacillus helveticus BD08, Lactobacillus plantarum BP06, Lactobacillus acidophilus BA05, Bifidobacterium animalis subsp. lactis BL03, Bifidobacterium breve BB02, Bifidobacterium animalis subsp. lactis BI04, and Streptococcus thermophilus BT01 (VSL#3 ® , lot number 909031, VSL Pharmaceuticals, Gaithersburg, MD, USA) , 8 weeks , Significant reduction in citrulline, TNF-ALFA, IL-6, and IL-12RA.

Techniques: Probiotics, Marker, Cell Counting

Journal: Applied and Environmental Microbiology

Article Title: Highly efficient CRISPR-Cas9 base editing in Bifidobacterium with bypass of restriction modification systems

doi: 10.1128/aem.01985-24

Figure Lengend Snippet: Disruption of a conserved bile acid biosynthetic pathway in different Bifidobacterium strains using the same editing plasmid. ( A ) Comparison of editing efficiencies using the cBEST2- bsh -PS1, cBEST3- bsh -PS1, and cBEST4- bsh -PS1 plasmids across Bifidobacterium strains NCIMB 8809, DSM 20219, and DSM 20088. Editing efficiencies for each experiment were expressed as (number of edited colonies / number of sequenced colonies) with data from independent transformations separated by commas. For transformations yielding less than five transformants, all transformants were sequenced. Data shaded orange were first presented in and reproduced here to facilitate comparison between the different constructs. ( B ) Editing window for all tested conditions. ( C ) Successful bsh (W22*) strains using the identical cBEST4- bsh -PS1 plasmid. Sequencing results confirmed the precise introduction of the stop codon due to successful C-to-T edits (red frames). ( D ) Biosynthesis pathway from taurocholic acid (TCA) to deoxycholic acid (DCA) by gut microbiota. Bile salt hydrolase ( bsh ) deconjugates TCA into cholic acid (CA), and bile acid-inducible operon ( bai ) converts CA into DCA. ( E ) Individual wild-type or bsh (W22*) strains cultured in TCA-supplemented MRSC broth for 24 h. ( F ) LC-MS/MS quantification of TCA and CA metabolites in indicated Bifidobacterium wild-type and bsh (W22*) strains. ( G ) Three Bifidobacterium wild-type or bsh (W22*) strains with Clostridium scindens DSM 5676 ( Clos .) co-cultures in TCA-supplemented MRSC broth for 24 h. ( H ) LC-MS/MS quantification of TCA, CA, and DCA metabolites in two co-cultures. n = 4, biological replicates; error bars, SEM. nt, no transformant obtained.

Article Snippet: Bifidobacterium strains and Clostridium scindens DSM 5676 were propagated in the Bioresource Collection and Research Center (BCRC) and cultured in NEOGEN Lactobacilli de Man, Rogosa, and Sharpe broth (LOT #US113980G) containing 0.05% wt/vol L-cysteine (MRSC) at 37°C under anaerobic conditions (80% N 2 , 10% CO 2 , 10% H 2 ) using Whitley DG250 anaerobic workstation.

Techniques: Disruption, Plasmid Preparation, Comparison, Construct, Sequencing, Cell Culture, Liquid Chromatography with Mass Spectroscopy